Publications

• Wang GL, Jiang B-H, Rue EA, and Semenza GL: Hypoxia-inducible factor 1 is a basic-helix-loop-helix-PAS heterodimer regulated by cellular O2 tension. Proc. Natl. Acad. Sci. USA 92, 5510-5514, 1995. Abstract
• Forsythe, JA, Jiang, B-H, Iyer, NV, Agani, F, Leung, SW, Koos, RD, and Semenza, GL: Activation of vascular endothelial growth factor gene transcription by hypoxia-inducible factor 1. Mol. Cell. Biol. 16, 4604-4613, 1996. Abstract
• Winograd, J, Reilly, MP, Roe, R, Lutz, J, Laughner, E, Xu, X, Hu, L, Asakura, T, Vander Kolk, C, Strandberg, JD, and Semenza, GL: Perinatal lethality and multiple craniofacial malformations in MSX2 transgenic mice. Hum. Mol. Genet. 6, 369-379, 1997. Abstract
• Iyer NV, Kotch LE, Agani F, Leung SW, Laughner E, Wegner R, Gassman M, Gearhart JD, Lawler AM, Yu AY, Semenza GL: Cellular and developmental control of O2 homeostasis by hypoxia-inducible factor 1 alpha. Genes Deve 12:149-162, 1998. Abstract
• Yu, AY, Shimoda, LA, Iyer, NV, Huso, DL, Sun, X, McWilliams, R, Beaty, T, Sham, JSK, Wiener, CM, Sylvester, JT, and Semenza, GL: Impaired physiological responses to chronic hypoxia in mice partially deficient for hypoxia-inducible factor 1alpha. J. Clin. Invest. 103, 691-696, 1999. Abstract
• Zhong, H, De Marzo, AM, Laughner, E, Lim, M, Hilton, DA, Zagzag, D, Buechler, P, Isaacs, WB, Semenza, GL, and Simons, JW: Overexpression of hypoxia-inducible factor 1alpha in common human cancers and their metastases. Cancer Res. 59, 5830-5835, 1999. Abstract
• Ravi, R, Mookerjee, B, Bhujwalla, ZM, Sutter, CH, Artemov, D, Zeng, Q, Dillehay, LE, Madan, A, Semenza, GL, and Bedi, A: Regulation of tumor angiogenesis by p53-induced degradation of hypoxia-inducible factor 1alpha. Genes Dev. 14, 34-44, 2000. Abstract
• Laughner, E, Taghavi, P, Chiles, K, Mahon, PC, and Semenza, GL : HER2 (neu) signaling increases the rate of hypoxia-inducible factor 1alpha (HIF-1alpha) synthesis: novel mechanism for HIF-1-mediated vascular endothelial growth factor expression. Mol. Cell. Biol. 21, 3995-4004, 2001. Abstract
• Semenza, GL: HIF-1, O2, and the 3 PHDs: how animal cells signal hypoxia to the nucleus. Cell 107, 1-3, 2001. Abstract
• Mahon, P C, Hirota, K, and Semenza, GL: FIH-1: a novel protein that interacts with HIF-1alpha and VHL to mediate repression of HIF-1 transcriptional activity. Genes Dev. 15, 2675-2686, 2001. Abstract
• Aebersold DM, Burri P, Beer KT, Laissue J, Djonov V, Greiner RH, and Semenza GL.  Expression of hypoxia-inducible factor 1a: a novel predictive and prognostic parameter in the radiotherapy of oropharyngeal cancer.  Cancer Res. 61, 2911-2916, 2001.
• Laughner E, Taghavi P, Chiles K, Mahon PC, and Semenza GL.  HER2 (neu) signaling increases the rate of hypoxia-inducible factor 1a (HIF-1a) synthesis: novel mechanism for HIF-1-mediated vascular endothelial growth factor expression.  Mol. Cell. Biol. 21, 3995-4004, 2001.
• Fukuda R, Hirota K, Fan F, Jung YD, Ellis LM, and Semenza GL.   IGF-1 induces HIF-1 mediated VEGF expression that is dependent on MAP kinase and PI-3-kinase signaling in colon cancer cells.  J. Biol. Chem. 277, 38205-38211, 2002.
• Cai Z, Manalo DJ, Wei G, Rodriguez ER, Fox-Talbot K, Lu H, Zweier JL, and Semenza GL.  Hearts from rodents exposed to intermittent hypoxia or erythropoietin are protected against ischemia-reperfusion injury.  Circulation 108: 79-85, 2003.
• Semenza GL.  Angiogenesis in ischemic and neoplastic disorders.  Annu. Rev. Med. 54: 17-28, 2003.
• Semenza GL.  Targeting HIF-1 for cancer therapy.  Nat. Rev. Cancer 3: 721-732, 2003.
• Kelly BD, Hackett SF, Hirota K, Oshima Y, Cai Z, Berg-Dixon S, Rowan A, Yan Z, Campochiaro PA, and Semenza GL.  Cell-type-specific regulation of angiogenic growth factor gene expression and induction of angiogenesis in non-ischemic tissue by a constitutively-active form of hypoxia-inducible factor 1.  Circ. Res.  93: 1074-1081, 2003.
• Cai Z, and Semenza GL.  Phosphatidylinositol-3-kinase signaling is required for erythropoietin-mediated  acute protection against myocardial ischemia-reperfusion injury.  Circulation  109: 2050-2053, 2004.
• Semenza GL.  Hydroxylation of HIF-1: oxygen sensing at the molecular level.  Physiology 19: 176-182, 2004.
• Manalo DJ, Rowan A, Lavoie T, Natarajan L, Kelly BD, Ye SQ, Garcia JGN, and Semenza GL.  Transcriptional regulation of vascular endothelial cell responses to hypoxia by HIF-1.  Blood 105: 661-671, 2005.
• Baek JH, Mahon PC, Oh J, Kelly B, Krishnamachary B, Pearson M, Chan DA, Giaccia AJ, and Semenza GL.  OS-9 interacts with hypoxia inducible factor 1a and prolyl hydroxylases to promote oxygen-dependent degradation of HIF-1a.  Mol. Cell 17: 503-512, 2005.
• Zagzag D, Krishnamachary B, Yee H, Okuyama H, Chiriboga L, Ali MA, Melamed J, and Semenza GL.  Stromal cell-derived factor 1a and CXCR4 expression in hemangioblastoma and renal clear cell carcinoma: von Hippel-Lindau loss-of-function induces expression of a ligand and its receptor.  Cancer Res. 65: 6178-6188, 2005.
• Resar JR, Roguin A, Voner J, Nasir K, Hennebry TA, Miller JM, Ingersoll R, Kasch LM, and Semenza GL.  Hypoxia inducible factor 1a polymorphism and coronary collaterals in patients with ischemic heart disease.  Chest 128: 787-791, 2005.
• Patel TH, Kimura H, Weiss CR, Semenza GL, and Hofmann LV.  Constitutively active HIF-1a improves perfusion and arterial remodeling in an endovascular model of limb ischemia.  Cardiovasc. Res. 68: 144-154, 2005.
• Cai Z, and Semenza GL.  PTEN activity is modulated during ischemia and reperfusion: involvement in the induction and decay of preconditioning.  Circ. Res. 97: 1351-1359, 2005.
• Krishnamachary B, Zagzag D, Nagasawa H, Rainey K, Okuyama H, Baek JH, and Semenza GL. Hypoxia-inducible factor-1-dependent repression of E-cadherin in von Hippel-Lindau tumor suppressor-null renal cell carcinoma mediated by TCF3, ZFHX1A, and ZFHX1B.  Cancer Res. 66: 2725-2731, 2006.
• Semenza GL, Shimoda LA, and Prabhakar NR.  Regulation of gene expression by hypoxia-inducible factor 1.  Novartis Found. Symp. 272: 2-8, 2006.
• Semenza GL.  Development of novel therapeutic strategies that target HIF-1.  Expert Opin. Ther. Targets 10: 267-280, 2006.
• Kim J-w, Tchernyshyov I, Semenza GL, and Dang CV.  HIF-1-mediated expression of pyruvate dehydrogenase kinase: a metabolic switch required for cellular adaptation to hypoxia.  Cell Metab. 3: 177-185, 2006.
• Okuyama H, Krishnamachary B, Zhou YF, Nagasawa  H, Bosch-Marce M, and Semenza  GL. Expression of vascular endothelial growth factor receptor 1 in bone marrow-derived mesenchymal cells is dependent on hypoxia-inducible factor 1.  J. Biol. Chem. 281: 15554-15563, 2006.
• Peng Y, Yuan G, Ramakrishnan D, Sharma SD, Bosch-Marce M, Kumar GK, Semenza GL, and Prabhakar NR. Heterozygous HIF-1a deficiency impairs carotid body-mediated systemic responses and reactive oxygen species generation in mice exposed to intermittent hypoxia.  J. Physiol. 577: 705-716, 2006.
• Shimoda LA, Fallon M, Pisarcik S, Wang J, Semenza GL. HIF-1 regulates hypoxic induction of NHE1 expression and alkalinization of intracellular pH in pulmonary arterial myocytes.  Am. J. Physiol.  291: 941-949, 2006.
• Liu YV, Baek JH, Zhang H, Diez R, Cole RN, and Semenza GL. RACK1 competes with HSP90 for binding to HIF-1a and is required for O2-independent and HSP90 inhibitor-induced degradation of HIF-1a. Mol. Cell 25: 207-217, 2007.
• Fukuda R, Zhang H, Kim J-w, Shimoda L, Dang CV, and Semenza GL. HIF-1 regulates cytochrome oxidase subunits to optimize efficiency of respiration in hypoxic cells. Cell 129: 111-122, 2007.
• Zhang H, Gao P, Fukuda R, Kumar G, Krishnamachary B, Zeller KI, Dang CV, and Semenza GL.  HIF-1 inhibits mitochondrial biogenesis and cellular respiration in VHL-deficient renal cell carcinoma by repression of C-MYC activity.  Cancer Cell 11: 407-420, 2007.
• Baek JH, Liu YV, McDonald KR, Wesley JB, Hubbi ME, Byun H, and Semenza GL.  Spermidine/spermine-N1-acetyltransferase 2 is an essential component of the ubiquitin ligase complex that regulates hypoxia-inducible factor 1a. J. Biol. Chem. 282: 23572-23580, 2007.
• Gao P, Zhang H, Dinavahi R, Li F, Xiang Y, Raman V, Bhujwalla ZM, Felsher DW, Cheng L, Pevsner J, Lee LA, Semenza GL, and Dang CV.  HIF-1-dependent anti-tumorigenic effect of antioxidants in vivo.  Cancer Cell 12: 230-238, 2007.
• Semenza GL. Oxygen-dependent regulation of mitochondrial respiration by hypoxia-inducible factor 1.  Biochem. J.  405: 1-9, 2007.
• Semenza GL. Life with oxygen. Science 318: 62-64, 2007.
• Semenza GL. Hypoxia-inducible factor 1 (HIF-1) pathway. Sci. STKE 2007 Oct 9;2007(407):cm8. [Sci. STKE (Connections Map in the Database of Cell Signaling), http://stke.sciencemag.org/cgi/cm/stkecm;CMP_19178 (2007).]
• Baek JH, Liu YV, McDonald KR, Wesley JB, Zhang H, and Semenza GL. Spermidine/spermine-N1-acetyltransferase-1 binds to hypoxia-inducible factor-1a (HIF-1a) and RACK1 and promotes ubiquitination and degradation of HIF-1a. J. Biol. Chem. 282: 33358-33366, 2007.
• Semenza GL. Vasculogenesis, angiogenesis, and arteriogenesis: mechanisms of blood vessel formation and remodeling. J. Cell. Biochem. 102: 840-847, 2007.
• Bosch-Marce M, Okuyama H, Wesley JB, Sarkar K, Kimura H, Liu YV, Zhang H, Strazza M, Rey S, Savino L, Zhou YF, McDonald KR, Na Y, Vandiver S, Rabi A, Shaked Y, Kerbel R, LaVallee T, and Semenza GL.  Effects of aging and HIF-1 activity on angiogenic cell mobilization and recovery of perfusion following limb ischemia. Circ. Res. 101:1310-1318, 2007.
• Liu YV, Hubbi ME, Pan F, McDonald KR, Mansharamani M, Cole RN, Liu JO, and Semenza GL. Calcineurin promotes hypoxia-inducible factor 1a expression by dephosphorylating RACK1 and blocking RACK1 dimerization.  J. Biol. Chem. 282: 37064-37073, 2007.
• Cai Z, Zhong H, Bosch-Marce M, Fox-Talbot K, Wang L, Wei C, Trush MA, and Semenza GL. Complete loss of ischemic preconditioning-induced cardioprotection in mice with partial deficiency of HIF-1a. Cardiovasc. Res. 77: 463-470, 2008.
• Semenza GL. Hypoxia-inducible factor 1 and cancer pathogenesis. IUBMB Life 60: 591-597, 2008.
• Semenza GL. Mitochondrial autophagy: life and breath of the cell. Autophagy 4 :534-536, 2008.
• Zhang H, Bosch-Marce M, Shimoda LA, Tan YS, Baek JH, Wesley JB, Gonzalez FJ, and Semenza GL. Mitochondrial autophagy is a HIF-1-dependent adaptive metabolic response to hypoxia. J. Biol. Chem. 283: 10892-10903, 2008.
• Zhang H, Qian DZ, Tan YS, Lee K, Gao P, Ren YR, Rey S, Hammers H, Chang D, Pili R, Dang CV, Liu JO, and Semenza GL. Digoxin and other cardiac glycosides inhibit HIF-1a synthesis and block tumor growth.  Proc. Natl. Acad. Sci. USA, in press.